Histološka, MRI i analiza genske ekspresije reorganizacijskih procesa u medijalnom (limbičkom) zidu ljudskog mozga tijekom razvitka

Opis projekta
The salient feature of the human fetal cortex is the presence of the subplate zone (SP), a prominent, synapse rich compartment composed of „waiting“ afferents, loosely arranged axons, synapses, growth cones, migratory neurons, glial cells and extraordinary amount of extracellular matric molecules (ECM). The medial (limbic) cortical regions (areas involved in emotion and memory functions) develop a different laminar structure in comparison with lateral cortical regions. Unlike the lateral cortex, where the SP is a major site of neurogenetic events, in the medial archicortex marginal zone (MZ) seems to be a key zone for early molecular neuronal interactions, synaptogenesis and patterning of growing pathways. The goal of this project is to correlate developmental reorganization of major cellular (cell bodies, growing axons) components of the SP and the MZ in developing medial wall of the human cerebrum (cingulum, hippocampus, amygdala) by combining immunochistochemistry, in situ hybridization, transcriptome analysis with in vivo/in vitro MRI. Histological analysis wiil be performed on postmortem human brains, ranging from 10 postconceptional weeks (POW) to 6.5 years, to analyze the expression patterns of different developmentally regulated proteins in relation to synaptic, laminar, neuronal, glial and ECM development. These results will be correlated with in vivo/in vitro MRI findings obtained in age-matched fetuses, infants and children. In addition, we will also analyze publicly available gene expression database (Kang et al., 2011) to investigate the relationship between structural changes and spatio-temporal expression of specific candidate genes. For the selected candidate genes we will perform in situ hybridization and immunocytochemistry in order to elucidate in which cells are these genes expressed. The obtained data will be used for setting the baseline for studying developmental abnormalities characteristic for diverse neurological disorders


Šifra projekta


Vrsta projekta

Vrsta financiranja
Kompetitivno projektno financiranje

HRZZ - Hrvatska zaklada za znanost

Detalji financiranja
 IP - Istraživački projekti

Ukupan iznos financiranja projekta
1.000.000,00 HRK

Trajanje projekta
01-09-2014 - 31-08-2018

Ustanova - uloga
Medicinski fakultet, Zagreb - Nositelj (1.000.000,00 HRK) [01-09-2014 - 31-08-2018]

Osoba - Ustanova - uloga [pocetak - zavrsetak]
Mario Vukšić (MBZ: 251416, CROSBI: 29769) - Medicinski fakultet, Zagreb - Voditelj projekta [01-09-2014 - 31-08-2018]