Višenamjensko očitavanje DNA/RNA sekundarne strukture molekularnim kemijskim senzorima

Opis projekta
Molecular recognition of DNA/RNA is of fundamental importance e.g. for gene transcription and regulation, protein synthesis or cell-division. Small molecules selectively targeting specific DNAs or RNAs are therefore highly interesting not only to understand such processes in more detail but also for potential medicinal applications, as sensors or probes for fluorescence based bioimaging as well as for analytical purposes in isolated biosamples. Proposal is based on the collaboration between I. Piantanida and Prof. Dr. C. Schmuck (Germany), joined results in leading chemistry journals (Chemistry, Eur. J; 2010 and 2012, J. Am. Chem. Soc. 2012; Org. Biomol. Chem. 2011, Bioorg. Med. Chem. Lett. 2008). We want to design, synthesize and study highly selective probes for the sensing of DNA/RNA with emphasis on the multi-purpose probes: single molecules able to simultaneously differentiate by several highly sensitive spectrometric methods between the most common secondary structures of ds- DNA/RNA. For increased affinity and selectivity, we plan to test new, more efficient fluorophores (cyanine, phenanthridine, fluorescent nucleobases-Piantanida group) combined with pyrrole-guanidinium cations (Schmuck group) and explore structural diversity of the compounds, by combinatorial approach (solid phase synthesis) based on peptide backbone carrying recognition units/fluorophores. The interaction with DNA/RNA will be studied by Piantanida group (UV/Vis, fluorescence, CD/LD spectroscopy, DSC/ITC microcalorimetry), screening of oligopeptide libraries by IRORI method and atomic force microscopy by Schmuck group. Proposal addresses CSF goals: a) support the collaboration with well-established international leaders, b) foster training of young researchers by work on the INTERNATIONAL research; c) expected results with commercializing potential (novel biochemical probes and/or drugs). The latter feature (c) will be studied within FP7-REGPOT “Innomol” Project at Ruđer Bošković Institute.

Akronim
DNA/RNA-MolSense

Šifra projekta
IP-2013-11-1477

Poziv
2013-11

Vrsta projekta
Znanstveno-istraživački

Vrsta financiranja
Kompetitivno projektno financiranje

Financijer
HRZZ - Hrvatska zaklada za znanost

Detalji financiranja
 IP - Istraživački projekti

Ukupan iznos financiranja projekta
987.922,00 HRK

Trajanje projekta
2014-09-01 - 2018-08-31

Ustanova - uloga
Institut "Ruđer Bošković", Zagreb - Nositelj (987.922,00 HRK)

Osoba - Ustanova - uloga [pocetak - zavrsetak]
Ivo Piantanida (MBZ: 202026, CROSBI: 15320) - Institut "Ruđer Bošković", Zagreb - Voditelj projekta [2014-09-01 - 2018-08-31]
Marijana Radić Stojković (MBZ: 239395, CROSBI: 22088) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Lidija-Marija Tumir (MBZ: , CROSBI: ) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Ivo Crnolatac (MBZ: 319642, CROSBI: 25948) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Biserka Žinić (MBZ: 95066, CROSBI: 30263) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Dijana Pavlović Saftić (MBZ: 305172, CROSBI: 1409) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Željka Ban (MBZ: 305225, CROSBI: 25705) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Renata Kobetić (MBZ: 150021, CROSBI: 21144) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Vilko Smrečki (MBZ: 190003, CROSBI: 14702) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Aleksandar Višnjevac (MBZ: 229664, CROSBI: 29240) - Institut "Ruđer Bošković", Zagreb - Suradnik [2014-09-01 - 2018-08-31]
Carsten Schmuck (MBZ: , CROSBI: ) - nema - Suradnik [2014-09-01 - 2018-08-31]
Tamara Šmidlehner (MBZ: 360230, CROSBI: 33983) - nema - Suradnik [2014-09-01 - 2018-08-31]